I definitely recommend you check all the usual suspects: power cycle your network switch and/or router, check the network cables, make sure you can 'ping' the OP2, etc.
FWIW, I had a similar problem on Saturday: I had PC Access up and running and connected to my OP2, and I was going room to room checking the sensors and their status. About halfway through, I lost my connection to the OP2. I thought I was in a Wifi deadspot, so I tried to 'repair' the connection (to no avail), I plugged into the ethernet jack in the room (no help), i moved back to the family room (still can't connect), I power cycled my network switches (still didn't work), tried a serial cable directly to the OP2 (still didn't connect), tried uninstall/reinstall of PCA (still didn't work), checked the IP address and encryption key (they were correct, but I still couldn't get in), and finally.. I power cycled the OP2.
everything worked fine after that.